Now that we’ve discussed the basic theory behind dose responses, lets apply them to GraphPad Prism. If your concentrations are very separated from each other, applying the logarithmic function to concentrations will allow better visualization of the shape of the curve by reducing dispersion of the data.
To obtain a more accurate representation, it is usually recommended to plot 5-10 concentrations distributed across the curve, so that you obtain the values that for the upper and lower “plateaus” as well as the transition between both of them. The assay used will probably illustrate the relationship between the amount of fluorescence generated and the concentration of the target, allowing you to convert simply by dividing by a constant. For example, you may measure the fluorescence of a sample, but would like to plot in concentration units. Additionally, Y values can be transformed into different units by multiplying or dividing by the appropriate constant.
Said response can result in a downward sigmoidal curve or an upward sigmoidal curve, representing the effect that the drug has on the system. But before we do that, let us dive a little deeper into the theory behind this analysis.Īs mentioned before, Y values are the responses measured at that dose (concentration) of the drug in question. Once the experiments have been performed, the data obtained and the curve plotted, a plethora of statistical analyses can be ran on to obtain greater insight on study, like the famous EC50 and IC50 values. Plotting these value usually results in a sigmoidal curve (or the dose-response curve) with a bottom and a top flat section, or “plateaus”. In a dose-response curve, the X values represent the drug concentrations or a function of concentrations (like the logarithm of concentrations) and the Y values represent the response that is under question. Today we will learn some of the basic concepts of Dose-Dependent responses, as well as a simple way to graph and analyze them using the GraphPad Prism software. Fortunately, this mathematical model is one of GraphPad Prism’s specialty.
But even a complex situation like this can be analyzed with the relatively simple approach of plotting X against Y values. 圖文：康恩宇(Robert Midence)/生醫所博士生 From a pharmacological perspective, the most classic version of a dose-response experiment would be the determining of a drugs effect on a biological system, like cells grown in vitro.
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